Monoclonal antibodies against ethacrynic acid high-sensitive Mg-ATPase.
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چکیده
منابع مشابه
Monoclonal Antibodies
SUMMARY Monoclonal Antibodies are identical antibodies and Can be generated in ultimated amounts by countinuos cultures of single antibody- secreting cells. These cell lines are produced by cell fusion of normal lymphocytes to cells of a myeloma tumor cell line, which confers on the antibody producing hybrid cell, immortality and the ability grow as atumor in animals. monoclonal antibodies c...
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It has been well established that monoclonal antibodies, since their initial description', play an important role on biological research . This technique has also been applied to studies on plant virus2·6> and this aspect was reviewed >. The basic characteristics of monoclonal antibodies are that (1) the antibody specifically reactive with one epitope can be obtained, (2) a hybridoma clone, onc...
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We produced two monoclonal hybridoma cell lines that secrete IgG immunoglobulins with high affinities (Kd = 7.3 to 8.0 X 10(-11) mol/L, Ka = 1.25 to 1.37 X 10(10) L/mol) for 125I-labeled human prostatic acid phosphatase (PAP), and that specifically bind this enzyme from human serum. The antibodies were produced in high titers in murine ascitic fluid (700 mg/L) and in cell-culture media (30 mg/L...
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We have investigated the functional role of the coated vesicle-uncoating ATPase (UA), a cognate heat shock protein (hsc70), in receptor-mediated endocytosis. A monoclonal antibody against bovine brain UA/hsc70 was generated that recognizes a 26 kDa proteolytic fragment harbouring the putative clathrin-binding site. In vitro, this antibody blocked the UA/hsc70-mediated release of clathrin from i...
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Background Leptospirosis is a zoonotic disease caused by pathogenic bacteria belonging to the genus Leptospira and more than 1 million cases occur worldwide annually. Several mammals may carry the agent, and rats are the most important source of human infection in urban settings. The Leptospira surface proteins have an important role during pathogen infection and some of these allow the differe...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1983
ISSN: 0021-5198
DOI: 10.1016/s0021-5198(19)64864-x